Visualizing Biomass Cell Wall Degrading Enzyme Complexes and Aggregates
نویسندگان
چکیده
منابع مشابه
Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail.
The small bacteriophage phi29 must penetrate the approximately 250-A thick external peptidoglycan cell wall and cell membrane of the Gram-positive Bacillus subtilis, before ejecting its dsDNA genome through its tail into the bacterial cytoplasm. The tail of bacteriophage phi29 is noncontractile and approximately 380 A long. A 1.8-A resolution crystal structure of gene product 13 (gp13) shows th...
متن کاملBacillus Subtilis Sj01 Produces Hemicellulose Degrading Multi-enzyme Complexes
Cellulose and hemicellulose account for a large portion of the world’s plant biomass. In nature, these polysaccharides are intertwined, forming complex materials that require multiple enzymes to degrade them. Multienzyme complexes (MECs) consist of a number of enzymes working in close proximity and synergistically to degrade complex substrates with higher efficiency than individual enzymes. The...
متن کاملThe Chlamydomonas cell wall degrading enzyme, lysin, acts on two substrates within the framework of the wall
The Chlamydomonas cell wall is a multilayered, extracellular matrix containing 20-25 proteins and glycoproteins, many of which are highly enriched in hydroxyproline. 80-90% of the wall protein is located in a crystalline portion of the wall that is soluble in sarkosyl-urea solutions as well as in chaotropic salts. Although the wall has no cellulose it contains a noncrystalline, highly insoluble...
متن کاملPlant cell wall degrading and remodeling proteins: current perspectives
Lignocellulose constitutes a raw material with considerable potential for the production of fermentable sugars and the generation of biofuels. In nature, lignocellulosic waste from forestry, agriculture and gardening acts as the preferred carbon source of a number of bacteria and fungi endowed with the required ligninolytic machinery. These hydrolytic activities could potentially be complemente...
متن کاملThermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production
Nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at Brazilian Cerrado and identified up to species level on the basis of the gene sequencing of 5.8S-ITS and 26S ribosomal DNA regions. Four species were identified: Kluyveromyces marxianus, Aspergillus niger, Aspergillus sydowii and Aspergillus fumigatus, and the isolates were screene...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2010
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927610059908